Experimental Setup | |
Tissue | Tissue atlas (Mergner et al., 2020) |
---|---|
Condition | Control conditions |
MS Instrument | Q Exactive HF |
MS/MS Search Parameters | |
Protein Database | Arabidopsis thaliana |
Decoy Strategy | Target-decoy |
FDR Threshold | 0.01 |
Search Algorithm(s) | MSFragger version 3.3 |
Precursor Mass Tolerance | Open search [-150; 500 Da] |
Protease | Trypsin |
Fixed Modifications | Carbamidomethylation (C) |
Variable Modifications | Oxidation (M) |
Other Information | |
Comments | Mass shift of 197.045 of Glu301 of elongation factor alpha 1 (eEF-1A) corresponding to glycerol phosphoryl ethanolamine (+ 197.045 Da), which is in line with previous reports (Whiteheart et al., 1989; Ransom et al., 1998). |