Experimental Setup | |
Tissue | Tissue atlas (Mergner et al., 2020) |
---|---|
Condition | Control conditions |
MS Instrument | Q Exactive HF |
MS/MS Search Parameters | |
Protein Database | Arabidopsis thaliana |
Decoy Strategy | Target-decoy |
FDR Threshold | 0.01 |
Search Algorithm(s) | MSFragger version 3.3 |
Precursor Mass Tolerance | Open search [-150; 500 Da] |
Protease | Trypsin |
Fixed Modifications | Carbamidomethylation (C) |
Variable Modifications | Oxidation (M) |
Other Information | |
Comments | Mass shift of 188.033 Da localized to Lys of the glycine decarboxylase complex proteins GDH1 and GDH2 – corresponding to the mass of the lipoyl cofactor known to bind these Lys. |