Publication Information
Zeng et al., 2018
Abstract
Mol Cell Proteomics. 2018 Mar;17(3):413-421. doi: 10.1074/mcp.RA117.000165. Epub
2017 Dec 13.
N-linked Glycan Micro-heterogeneity in Glycoproteins of Arabidopsis.
Zeng W(1)(2), Ford KL(1), Bacic A(1), Heazlewood JL(3)(4).
Author information:
(1)From the ‡ARC Centre of Excellence in Plant Cell Walls, School of
BioSciences, The University of Melbourne, Melbourne, VIC 3010, Australia.
(2)§Sino-Australia Plant Cell Wall Research Centre, State Key Laboratory of
Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A&F
University, Lin'an, China.
(3)From the ‡ARC Centre of Excellence in Plant Cell Walls, School of
BioSciences, The University of Melbourne, Melbourne, VIC 3010, Australia;
jheazlewood@unimelb.edu.au.
(4)¶Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley,
California 94702.
N-glycosylation is one of the most common protein post-translational
modifications in eukaryotes and has a relatively conserved core structure
between fungi, animals and plants. In plants, the biosynthesis of N-glycans has
been extensively studied with all the major biosynthetic enzymes characterized.
However, few studies have applied advanced mass spectrometry to profile intact
plant N-glycopeptides. In this study, we use hydrophilic enrichment,
high-resolution tandem mass spectrometry with complementary and triggered
fragmentation to profile Arabidopsis N-glycopeptides from microsomal membranes
of aerial tissues. A total of 492 N-glycosites were identified from 324
Arabidopsis proteins with extensive N-glycan structural heterogeneity revealed
through 1110 N-glycopeptides. To demonstrate the precision of the approach, we
also profiled N-glycopeptides from the mutant (xylt) of
β-1,2-xylosyltransferase, an enzyme in the N-glycan biosynthetic pathway. This
analysis represents the most comprehensive and unbiased collection of
Arabidopsis N-glycopeptides revealing an unsurpassed level of detail on the
micro-heterogeneity present in N-glycoproteins of Arabidopsis. Data are
available via ProteomeXchange with identifier PXD006270.
© 2018 by The American Society for Biochemistry and Molecular Biology, Inc.
DOI: 10.1074/mcp.RA117.000165
PMCID: PMC5836367
PMID: 29237727 [Indexed for MEDLINE]
