Publication Information
Dogra et al. 2019
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Abstract
Nat Commun. 2019 Jun 27;10(1):2834. doi: 10.1038/s41467-019-10760-6.
Oxidative post-translational modification of EXECUTER1 is required for singlet
oxygen sensing in plastids.
Dogra V(1), Li M(1)(2), Singh S(1), Li M(1)(2), Kim C(3)(4).
Author information:
(1)Shanghai Center for Plant Stress Biology and Center of Excellence in
Molecular Plant Sciences, Chinese Academy of Sciences, 200032, Shanghai, China.
(2)University of the Chinese Academy of Sciences, 100049, Beijing, China.
(3)Shanghai Center for Plant Stress Biology and Center of Excellence in
Molecular Plant Sciences, Chinese Academy of Sciences, 200032, Shanghai, China.
chanhongkim@sibs.ac.cn.
(4)University of the Chinese Academy of Sciences, 100049, Beijing, China.
chanhongkim@sibs.ac.cn.
Environmental information perceived by chloroplasts can be translated into
retrograde signals that alter the expression of nuclear genes. Singlet oxygen
(1O2) generated by photosystem II (PSII) can cause photo-oxidative damage of
PSII but has also been implicated in retrograde signaling. We previously
reported that a nuclear-encoded chloroplast FtsH2 metalloprotease coordinates
1O2-triggered retrograde signaling by promoting the degradation of the EXECUTER1
(EX1) protein, a putative 1O2 sensor. Here, we show that a 1O2-mediated
oxidative post-translational modification of EX1 is essential for initiating
1O2-derived signaling. Specifically, the Trp643 residue in DUF3506 domain of EX1
is prone to oxidation by 1O2. Both the substitution of Trp643 with
1O2-insensitive amino acids and the deletion of the DUF3506 domain abolish the
EX1-mediated 1O2 signaling. We thus provide mechanistic insight into how EX1
senses 1O2 via Trp643 located in the DUF3506 domain.
DOI: 10.1038/s41467-019-10760-6
PMCID: PMC6597547
PMID: 31249292 [Indexed for MEDLINE]
Conflict of interest statement: The authors declare no competing interests.
