Ubiquitination Membrane Proteins
Ubiquitination in Arabidopsis thaliana
771 modifications in 750 peptides, found in 1184 proteins
Experiment Details
Exp 120
| Experimental Setup | |
| Tissue | 8-day old seedlings |
|---|---|
| Condition | Control conditions |
| PTM Enrichment | PTMScan® Ubiquitin Remnant Motif K-ε-GG antibody |
| MS Instrument | Orbitrap Fusion |
| MS/MS Search Parameters | |
| Protein Database | TAIR10 + in-house contaminants/constructs |
| Decoy Strategy | Target-decoy |
| FDR Threshold | Peptide level false discovery rate was 0.025 (Table S1) |
| Search Algorithm(s) | MASCOT (version 2.4.1) |
| Precursor Mass Tolerance | 10 ppm |
| Protease | Trypsin |
| Fixed Modifications | Carbamidomethylation (C) |
| Variable Modifications | GlyGly (K) |
| Oxidation (M) | |
| Other Information | |
| Comments | Table S1. Peptides shown were filtered for the ubiquitin remnant diGly and were observed at least twice with a minimal Mascot score of 20. |
Publication Information
Grubb et al., 2021
PubMed ID: 33585938
ProteomeXchange: PXD021992
Abstract
Plant Physiol. 2021 Apr 23;185(4):1483-1488. doi: 10.1093/plphys/kiab023. Large-scale identification of ubiquitination sites on membrane-associated proteins in Arabidopsis thaliana seedlings. Grubb LE(1)(2), Derbyshire P(2), Dunning KE(1), Zipfel C(2)(3), Menke FLH(2), Monaghan J(1)(2). Author information: (1)Department of Biology, Queen's University, Kingston, Canada. (2)The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK. (3)Department of Plant and Microbial Biology, Zurich-Basel Plant Science Center, University of Zurich, Zurich, Switzerland. An analysis of the identification of ubiquitination sites on proteins found at the cell periphery, including over 100 protein kinases. DOI: 10.1093/plphys/kiab023 PMCID: PMC8133621 PMID: 33585938 [Indexed for MEDLINE]