Publication Information
Yin et al., 2017
Abstract
Front Plant Sci. 2017 Jan 26;8:58. doi: 10.3389/fpls.2017.00058. eCollection
2017.
Bicarbonate Induced Redox Proteome Changes in Arabidopsis Suspension Cells.
Yin Z(1), Balmant K(2), Geng S(2), Zhu N(3), Zhang T(2), Dufresne C(4), Dai
S(5), Chen S(3).
Author information:
(1)Plant Molecular and Cellular Biology Program, Department of Biology, Genetics
Institute, University of FloridaGainesville, FL, USA; Key Laboratory of
Saline-alkali Vegetation Ecology Restoration in Oil Field, Alkali Soil Natural
Environmental Science Center, Ministry of Education, Northeast Forestry
UniversityHarbin, China.
(2)Plant Molecular and Cellular Biology Program, Department of Biology, Genetics
Institute, University of Florida Gainesville, FL, USA.
(3)Plant Molecular and Cellular Biology Program, Department of Biology, Genetics
Institute, University of FloridaGainesville, FL, USA; Interdisciplinary Center
for Biotechnology Research, University of FloridaGainesville, FL, USA.
(4)Thermo Fisher Scientific West Palm Beach, FL, USA.
(5)Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field,
Alkali Soil Natural Environmental Science Center, Ministry of Education,
Northeast Forestry University Harbin, China.
Climate change as a result of increasing atmospheric CO2 affects plant growth
and productivity. CO2 is not only a carbon donor for photosynthesis but also an
environmental signal that can perturb cellular redox homeostasis and lead to
modifications of redox-sensitive proteins. Although redox regulation of protein
functions has emerged as an important mechanism in several biological processes,
protein redox modifications and how they function in plant CO2 response remain
unclear. Here a new iodoTMTRAQ proteomics technology was employed to analyze
changes in protein redox modifications in Arabidopsis thaliana suspension cells
in response to bicarbonate (mimic of elevated CO2) in a time-course study. A
total of 47 potential redox-regulated proteins were identified with functions in
carbohydrate and energy metabolism, transport, ROS scavenging, cell structure
modulation and protein turnover. This inventory of previously unknown redox
responsive proteins in Arabidopsis bicarbonate responses lays a foundation for
future research toward understanding the molecular mechanisms underlying plant
CO2 responses.
DOI: 10.3389/fpls.2017.00058
PMCID: PMC5266719
PMID: 28184230
