Publication Information
Lawrence et al., 2020
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Abstract
Int J Mol Sci. 2020 Mar 1;21(5):1688. doi: 10.3390/ijms21051688.
S-Nitroso-Proteome Revealed in Stomatal Guard Cell Response to Flg22.
Lawrence SR 2nd(1)(2), Gaitens M(2), Guan Q(2), Dufresne C(3), Chen S(1)(2)(4).
Author information:
(1)Plant Molecular and Cellular Biology Program, University of Florida,
Gainesville, FL 32610, USA.
(2)Department of Biology, University of Florida Genetics Institute, Gainesville,
FL 32611, USA.
(3)Thermo Fisher Scientific, 1400 Northpoint Parkway, West Palm Beach, FL 33407,
USA.
(4)Proteomics and Mass Spectrometry, Interdisciplinary Center for Biotechnology
Research, University of Florida, Gainesville, FL 32610, USA.
Nitric oxide (NO) plays an important role in stomata closure induced by
environmental stimuli including pathogens. During pathogen challenge, nitric
oxide (NO) acts as a second messenger in guard cell signaling networks to
activate downstream responses leading to stomata closure. One means by which
NO's action is achieved is through the posttranslational modification of
cysteine residue(s) of target proteins. Although the roles of NO have been well
studied in plant tissues and seedlings, far less is known about NO signaling
and, more specifically, protein S-nitrosylation (SNO) in stomatal guard cells.
In this study, using iodoTMTRAQ quantitative proteomics technology, we analyzed
changes in protein SNO modification in guard cells of reference plant
Arabidopsis thaliana in response to flg22, an elicitor-active peptide derived
from bacterial flagellin. A total of 41 SNO-modified peptides corresponding to
35 proteins were identified. The proteins cover a wide range of functions,
including energy metabolism, transport, stress response, photosynthesis, and
cell-cell communication. This study creates the first inventory of previously
unknown NO responsive proteins in guard cell immune responses and establishes a
foundation for future research toward understanding the molecular mechanisms and
regulatory roles of SNO in stomata immunity against bacterial pathogens.
DOI: 10.3390/ijms21051688
PMCID: PMC7084773
PMID: 32121556 [Indexed for MEDLINE]
Conflict of interest statement: The authors declare no conflict of interest.
