Publication Information
Mergner et al., 2020
Abstract
Nature. 2020 Mar;579(7799):409-414. doi: 10.1038/s41586-020-2094-2. Epub 2020
Mar 11.
Mass-spectrometry-based draft of the Arabidopsis proteome.
Mergner J(1), Frejno M(1), List M(2), Papacek M(3), Chen X(4), Chaudhary A(4),
Samaras P(1), Richter S(5), Shikata H(6)(7), Messerer M(8), Lang D(8), Altmann
S(9), Cyprys P(10), Zolg DP(1), Mathieson T(11), Bantscheff M(11), Hazarika
RR(12)(13), Schmidt T(1), Dawid C(14), Dunkel A(14), Hofmann T(14), Sprunck
S(10), Falter-Braun P(9)(15), Johannes F(12)(13), Mayer KFX(8)(16), Jürgens
G(5), Wilhelm M(1), Baumbach J(2), Grill E(3), Schneitz K(4), Schwechheimer
C(6), Kuster B(17)(18)(19).
Author information:
(1)Chair of Proteomics and Bioanalytics, Technical University of Munich (TUM),
Freising, Germany.
(2)Chair of Experimental Bioinformatics, Technical University of Munich (TUM),
Freising, Germany.
(3)Chair of Botany, Technical University of Munich (TUM), Freising, Germany.
(4)Plant Developmental Biology, Technical University of Munich (TUM), Freising,
Germany.
(5)Center for Plant Molecular Biology, University of Tübingen, Tübingen,
Germany.
(6)Chair of Plant Systems Biology, Technical University of Munich (TUM),
Freising, Germany.
(7)Devision of Plant Environmental Responses, National Institute for Basic
Biology, Okazaki, Japan.
(8)Plant Genome and Systems Biology, Helmholtz Center Munich, German Research
Center for Environmental Health, Munich-Neuherberg, Germany.
(9)Institute of Network Biology (INET), Helmholtz Center Munich, German Research
Center for Environmental Health, Munich-Neuherberg, Germany.
(10)Cell Biology and Plant Biochemistry, University of Regensburg, Regensburg,
Germany.
(11)Cellzome GmbH, Heidelberg, Germany.
(12)Population Epigenetics and Epigenomics, Technical University of Munich
(TUM), Freising, Germany.
(13)Institute of Advanced Study (IAS), Technical University of Munich (TUM),
Freising, Germany.
(14)Chair of Food Chemistry and Molecular Sensory Science, Technical University
of Munich (TUM), Freising, Germany.
(15)Chair of Microbe-Host Interactions, Ludwigs-Maximilians-University (LMU),
Munich, Germany.
(16)Plant Genome Biology, Technical University of Munich (TUM), Freising,
Germany.
(17)Chair of Proteomics and Bioanalytics, Technical University of Munich (TUM),
Freising, Germany. kuster@tum.de.
(18)Institute of Advanced Study (IAS), Technical University of Munich (TUM),
Freising, Germany. kuster@tum.de.
(19)Bavarian Biomolecular Mass Spectrometry Center (BayBioMS), Technical
University of Munich (TUM), Freising, Germany. kuster@tum.de.
Comment in
Sci Data. 2020 Oct 9;7(1):334.
Plants are essential for life and are extremely diverse organisms with unique
molecular capabilities1. Here we present a quantitative atlas of the
transcriptomes, proteomes and phosphoproteomes of 30 tissues of the model plant
Arabidopsis thaliana. Our analysis provides initial answers to how many genes
exist as proteins (more than 18,000), where they are expressed, in which
approximate quantities (a dynamic range of more than six orders of magnitude)
and to what extent they are phosphorylated (over 43,000 sites). We present
examples of how the data may be used, such as to discover proteins that are
translated from short open-reading frames, to uncover sequence motifs that are
involved in the regulation of protein production, and to identify
tissue-specific protein complexes or phosphorylation-mediated signalling events.
Interactive access to this resource for the plant community is provided by the
ProteomicsDB and ATHENA databases, which include powerful bioinformatics tools
to explore and characterize Arabidopsis proteins, their modifications and
interactions.
DOI: 10.1038/s41586-020-2094-2
PMID: 32188942 [Indexed for MEDLINE]
