Publication Information
Liu et al., 2015
Abstract
Sci Rep. 2015 Feb 27;5:8625. doi: 10.1038/srep08625.
Proteomic identification of early salicylate- and flg22-responsive
redox-sensitive proteins in Arabidopsis.
Liu P(1), Zhang H(2), Yu B(3), Xiong L(4), Xia Y(5).
Author information:
(1)1] Department of Biology, Hong Kong Baptist University, Kowloon, Hong Kong
[2] Biological and Environmental Sciences and Engineering Division, King
Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
(2)Biosciences Core Laboratory, King Abdullah University of Science and
Technology, Thuwal, Saudi Arabia.
(3)Department of Biology, Hong Kong Baptist University, Kowloon, Hong Kong.
(4)Biological and Environmental Sciences and Engineering Division, King Abdullah
University of Science and Technology, Thuwal, Saudi Arabia.
(5)1] Department of Biology, Hong Kong Baptist University, Kowloon, Hong Kong
[2] Partner State Key Laboratory of Agrobiotechnology, Chinese University of
Hong Kong, Shatin, Hong Kong.
Accumulation of reactive oxygen species (ROS) is one of the early defense
responses against pathogen infection in plants. The mechanism about the initial
and direct regulation of the defense signaling pathway by ROS remains elusive.
Perturbation of cellular redox homeostasis by ROS is believed to alter functions
of redox-sensitive proteins through their oxidative modifications. Here we
report an OxiTRAQ-based proteomic study in identifying proteins whose cysteines
underwent oxidative modifications in Arabidopsis cells during the early response
to salicylate or flg22, two defense pathway elicitors that are known to disturb
cellular redox homeostasis. Among the salicylate- and/or flg22-responsive
redox-sensitive proteins are those involved in transcriptional regulation,
chromatin remodeling, RNA processing, post-translational modifications, and
nucleocytoplasmic shuttling. The identification of the
salicylate-/flg22-responsive redox-sensitive proteins provides a foundation from
which further study can be conducted toward understanding biological
significance of their oxidative modifications during the plant defense response.
DOI: 10.1038/srep08625
PMCID: PMC4342551
PMID: 25720653 [Indexed for MEDLINE]
