Publication Information
Engelsberger and Schulze, 2012
No external accession available
Abstract
Plant J. 2012 Mar;69(6):978-95. doi: 10.1111/j.1365-313X.2011.04848.x. Epub 2012
Jan 20.
Nitrate and ammonium lead to distinct global dynamic phosphorylation patterns
when resupplied to nitrogen-starved Arabidopsis seedlings.
Engelsberger WR(1), Schulze WX.
Author information:
(1)Max Planck Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1,
14476 Golm, Germany.
Nitrogen is an essential macronutrient for plant growth and development.
Inorganic nitrogen and its assimilation products control various metabolic,
physiological and developmental processes. Although the transcriptional
responses induced by nitrogen have been extensively studied in the past, our
work here focused on the discovery of candidate proteins for regulatory events
that are complementary to transcriptional changes. Most signaling pathways
involve modulation of protein abundance and/or activity by protein
phosphorylation. Therefore, we analyzed the dynamic changes in protein
phosphorylation in membrane and soluble proteins from plants exposed to rapid
changes in nutrient availability over a time course of 30 min. Plants were
starved of nitrogen and subsequently resupplied with nitrogen in the form of
nitrate or ammonium. Proteins with maximum change in their phosphorylation level
at up to 5 min after nitrogen resupply (fast responses) included GPI-anchored
proteins, receptor kinases and transcription factors, while proteins with
maximum change in their phosphorylation level after 10 min of nitrogen resupply
(late responses) included proteins involved in protein synthesis and
degradation, as well as proteins with functions in central metabolism and
hormone metabolism. Resupply of nitrogen in the form of nitrate or ammonium
resulted in distinct phosphorylation patterns, mainly of proteins with signaling
functions, transcription factors and transporters.
© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd.
DOI: 10.1111/j.1365-313X.2011.04848.x
PMCID: PMC3380553
PMID: 22060019 [Indexed for MEDLINE]
