Publication Information
Wang et al., 2012
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Abstract
J Proteome Res. 2012 Apr 6;11(4):2301-15. doi: 10.1021/pr2010764. Epub 2012 Feb
29.
A comprehensive differential proteomic study of nitrate deprivation in
Arabidopsis reveals complex regulatory networks of plant nitrogen responses.
Wang X(1), Bian Y, Cheng K, Zou H, Sun SS, He JX.
Author information:
(1)School of Life Sciences, Tsinghua University, Beijing 100084, China.
Nitrogen (N) is an important nutrient and signal for plant growth and
development. However, to date, our knowledge of how plants sense and transduce
the N signals is very limited. To better understand the molecular mechanisms of
plant N responses, we took two-dimensional gel-based proteomic and
phosphoproteomic approaches to profile the proteins with abundance and
phosphorylation state changes during nitrate deprivation and recovery in the
model plant Arabidopsis thaliana. After 7-day-old seedlings were N-deprived for
up to 48 h followed by 24 h recovery, a total of 170 and 38 proteins were
identified with significant changes in abundance and phosphorylation state,
respectively. Bioinformatic analyses implicate these proteins in diverse
cellular processes including N and protein metabolisms, photosynthesis,
cytoskeleton, redox homeostasis, and signal transduction. Functional studies of
the selected nitrate-responsive proteins indicate that the proteasome regulatory
subunit RPT5a and the cytoskeleton protein Tubulin alpha-6 (TUA6) play important
roles in plant nitrate responses by regulating plant N use efficiency (NUE) and
low nitrate-induced anthocyanin biosynthesis, respectively. In conclusion, our
study provides novel insights into plant responses to nitrate at the proteome
level, which are expected to be highly useful for dissecting the N response
pathways in higher plants and for improving plant NUE.
DOI: 10.1021/pr2010764
PMID: 22329444 [Indexed for MEDLINE]
