Publication Information
Umezawa et al., 2009
No external accession available
Abstract
Proc Natl Acad Sci U S A. 2009 Oct 13;106(41):17588-93. doi:
10.1073/pnas.0907095106. Epub 2009 Sep 29.
Type 2C protein phosphatases directly regulate abscisic acid-activated protein
kinases in Arabidopsis.
Umezawa T(1), Sugiyama N, Mizoguchi M, Hayashi S, Myouga F, Yamaguchi-Shinozaki
K, Ishihama Y, Hirayama T, Shinozaki K.
Author information:
(1)Gene Discovery Research Team, RIKEN Plant Science Center, 3-1-1 Kouyadai,
Tsukuba, Ibaraki 305-0074, Japan.
Comment in
Plant Signal Behav. 2010 Feb;5(2):160-3.
Abscisic acid (ABA) signaling is important for stress responses and
developmental processes in plants. A subgroup of protein phosphatase 2C (group A
PP2C) or SNF1-related protein kinase 2 (subclass III SnRK2) have been known as
major negative or positive regulators of ABA signaling, respectively. Here, we
demonstrate the physical and functional linkage between these two major
signaling factors. Group A PP2Cs interacted physically with SnRK2s in various
combinations, and efficiently inactivated ABA-activated SnRK2s via
dephosphorylation of multiple Ser/Thr residues in the activation loop. This step
was suppressed by the RCAR/PYR ABA receptors in response to ABA. However the
abi1-1 mutated PP2C did not respond to the receptors and constitutively
inactivated SnRK2. Our results demonstrate that group A PP2Cs act as
'gatekeepers' of subclass III SnRK2s, unraveling an important regulatory
mechanism of ABA signaling.
DOI: 10.1073/pnas.0907095106
PMCID: PMC2754379
PMID: 19805022 [Indexed for MEDLINE]
Conflict of interest statement: The authors declare no conflict of interest.
