Publication Information
Wang et al., 2009
No external accession available
Abstract
J Proteomics. 2009 Jul 21;72(5):831-5. doi: 10.1016/j.jprot.2009.03.006. Epub
2009 Mar 31.
A simple and effective method for detecting phosphopeptides for phosphoproteomic
analysis.
Wang Z(1), Dong G, Singh S, Steen H, Li J.
Author information:
(1)Department of Biochemistry and Molecular Biology, Mississippi State
University, Mississippi State, MS, USA.
An immuno-dot blot method for detecting phosphopeptides is described. This
method allows detection of phosphopeptides prior to analysis by mass
spectrometry, and does not require specialized equipment. Further, this method
allows peptides containing a given phosphoamino acid to be selectively detected
by using phosphoamino acid-specific antibody. This phosphopeptide assay should
be useful for researchers who do not have direct access to the mass
spectrometers to prepare high quality samples by optimizing phosphopeptide
enrichment procedures prior to mass spectrometry analysis.
DOI: 10.1016/j.jprot.2009.03.006
PMID: 19341826 [Indexed for MEDLINE]
