This project focusses on identifying novel factors downstream of the TMO5/LHW complex and understanding how the known players interact to control common or distinct downstream events. This is done using both forward genetics and reverse genetics approaches.
As many cell files need to be formed during vascular development, it is crucial to understand how plants control the orientations of their cell divisions. It this project we aim to understand this by using cell biology and chemical genetics.
It is clear the TMO5/LHW have a role in controlling vascular cell proliferation in higher plants. In this project, we want to understand if these factors are present in lower plants and if so, what the ancestral function of these transcription factors could be.
Over the past few years, we have been using this technology to identify interaction partners for the developmental regulators we are studying. We hereby provide a detailed protocol and several examples of publication in which this technology was successfully applied.