"Elucidating the transcriptional networks underlying hormone-mediated immunity in Arabidopsis"

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Monday 03 June 2019, 14:00 - 15:30

 
ABSTRACT

The phyotohormones, jasmonic acid (JA) and salicylic acid (SA) are central regulators of plant immunity that mediate defense against different types of attackers through the expression of broadly different sets of defense response genes. To obtain a detailed understanding of the dynamics and architecture of the transcriptional regulatory networks that underlie the JA- and SA-responses we have used two different, yet complementary, genomics approaches in the model plant Arabidopsis thaliana. First, we performed a high-resolution RNA-seq time series analysis of JA- and SA-treated leaves to obtain a detailed temporal map of transcriptional reprogramming induced by JA and SA over a 16-h timecourse. Analysis of this large-scale, information-rich data set allowed us to build dynamic regulatory maps of the JA and SA response, identify gene regulatory modules, and infer novel defense regulators; we have predicted and validated the roles of several different transcription factors (TFs) in JA/SA-mediated immunity. In the second approach we profiled the Arabidopsis chromatin accessibility landscape by sequencing ATAC-seq libraries prepared from seedlings treated with JA, SA or a mock control. These data allow us to delineate regions of accessible chromatin on a genome-wide scale and link these with JA- and SA-mediated changes in gene expression, while digital genomic footprinting suggests TF occupancy within the regulatory DNA of target genes; this information provides a direct and empirical approach for inferring transcriptional networks that may regulate these hormone responses.

 

 

 

 

 

 

Location Jozef Schell Seminar Room
Contact Dr Richard Hickman
Plant-Microbe Interactions
Department of Biology
Faculty of Science
Utrecht University
THE NETHERLANDS