“Cyclic Nucleotide-Gated Channels in Plants - Insights from Suppressor Screens of Two Lesion Mimic Mutants, cpr22 and dnd1; from pathogen resistance to structure function analysis –“

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Tuesday 26 September 2017, 11:00 - 12:30

 
ABSTRACT

Ca2+ signaling is central to many aspects of plant physiology; however, the channels involved in mediating Ca2+ fluxes in plants remain largely elusive. This is at least partially due to the absence of plant homologs to many of the well-characterized Ca2+ channels present in animals. Instead, plants have expanded families of ligand-gated, non-specific cation channels such as the cyclic nucleotide-gated channels (CNGCs) and glutamate receptor-like channels (GLRs), which are hypothesized to provide Ca2+ channel function in plants. The 20 member CNGC family represents one of two large families of putative Ca2+ channels in Arabidopsis, and various isoforms have been implicated in plant development and stress-response signaling, including immunity. Among Arabidopsis CNGC mutants, cpr22 and dnd1 are the best characterized mutants that show similar autoimmunity phenotypes such as constitutive expression of PR genes and heightened accumulation of salicylic acid. To understand the regulation of plant CNGCs and their signal transduction, we have conducted extensive characterization and suppressor screens of these two mutants. In this seminar, I will present the insights we have gained from our CNGC research program and discuss our current research direction.

Selected related articles:

  • DeFalco, T.A., Moeder, W., Yoshioka, K. (2016) Opening the gates: Insights into cyclic nucleotide-gated channel-mediated signaling. Trends Plant Sci 11: 903-906.
  • DeFalco, T.A., Marshall, C.B., Munro, K., Kang, H.G., Moeder, W., Ikura, M., Snedden, W.A., Yoshioka, K. (2016) Multiple calmodulin-binding sites positively and negatively regulate Arabidopsis CYCLIC NUCLEOTIDE-GATED CHANNEL 12. Plant Cell 28:1738-1751.
  • DeFalco, T,A., Toyota, M., Moeder, W., Gilroy, S., Yoshioka, K. (2017) Using GCaMP3 To Study Ca2+ Signaling In Nicotiana Species Plant Cell Physiol. 58:1173-1184.
  • Chin, K., DeFalco, T., Moeder, W., Yoshioka, K. (2013) The Arabidopsis cyclic nucleotide-gated ion channels, AtCNGC2 and AtCNGC4 work in the same signaling pathway to regulate pathogen defense and floral transition. Plant Physiol. 163:611-624.
  • Abdel Hamid, H., Chin K., Moeder, W., Shahinas, D., Gupta, D., Yoshioka, K. (2013) A suppressor screen of chimeric AtCNGC11/12 reveals residues important for intra-subunit interaction of cyclic nucleotide gated ion channels. Plant Physiol. 162:1681-1693.
  • Moeder, W., Ung, H., Urquhart, W. and Yoshioka, K. (2011) The role of cyclic nucleotide-gated ion channels in plant immunity. Molecular Plant 4:442-452.
  • Yoshioka, K., Moeder, W., Kang, H-G., Kachroo,P., Masmoudi,K., Berkowitz,G. and Klessig, D.F. (2006) The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 activates multiple pathogen resistance responses. Plant Cell 18:747-763

 

Location Jozef Schell Seminar Room
Contact Prof Keiko Yoshioka
Department of Cell & Systems Biology
University of Toronto
Toronto
CANADA