Maize transformation

The CGEF operates a maize transformation platform that can be accessed by academic labs and companies.

For more information about our maize transformation platform, practicalities and pricing, click here. Our platform is based on Agrobacterium tumefaciens-mediated transformation of immature embryos of the inbred line B104. Most of the constructs we handle are related to CRISPR. With our experience and in collaboration with the Jacobs lab, we can also help other labs with construct design, cloning and downstream analysis of new alleles. We also work closely together with the PSB TAP-platform for an AP/MS-based interactomics service in maize in either whole plants or callus. Upon request and depending on the scientific questions, maize lines can be analyzed on the automated phenotyping platform, PHENOVISION, in collaboration with the Inzé/Nelissen lab.

We are also developing particle bombardment-based methods for transformation of other genotypes such as H99; homologous recombination; and DNA-free CRISPR delivery.