for C-terminal GFP fusions, the stopcodon was removed, for N-terminal GFP fusions, primers were ordered including the stopcodon.
MIPS number Forward primer (F) Reverse primer (R) LENGTH
Yeast genes
At1g13350 Y1F ATGGTGAGTGACAAGCATGTAGAATC Y1R TCTTGCCCGTGATGAATGGG 2265
At1g73450 Y2F ATGGCAGACCAAAGCTCTGTTG Y2R TAGCAGAGATTGGCTCGTATGGG 3468
At1g73460 Y3F ATGACGGACCAGAGCTCTGTTG Y3R TAGCAGAGATTGGTTCGTATGGATATG 3510
At2g40120 Y4F ATGGCGGTTGATGTTAAATCTGTTC Y4R TATTGTAAGATGAAGAAGAAGAGAGCC 1713
At3g25840 Y5F ATGGCGAACGATAAGCAGATCG Y5R TCTTGCCAGTGATGAATGGGTG 2799
At3g17750 Y6F ATGATTTTCCATGGATCCAAAGG Y6R TGGCAGATATGGGTTCGTAGGG 3765
At3g53640 Y7F ATGGTGAGCGATAAGATAGTAGAATCAAG Y7R idem as Y1R 1929
At3g15220 Y18F ATGGACGATGTTGCGGGTT Y18R AAGATCACGTGATGATTGGCCT 2070
At3g23310 Y45F ATGGATACTGCCAGAGCATGGC Y45R GGATGTGGCATTGTCGAAACG 1704
At4g14350 Y46F ATGCTAGAGAAAAAATTAGCTGCTGC Y46R CCCGGATGAGCTCGACTTACTAC 1425
At3g21700 Y49F ATGGCTCAATCGTGTCTTAAAATTG Y49R GAAGTCGATGATTGGTTCTCCG 876
At5g54840 Y50F ATGGCCGAGAAAGTCCATGAG Y50R GGAATCGTAGTCGATAATGGGTTCAC 864
At3g13530 Y47F ATGGCGCGGCAAATGAC Y47R CAATATTGTGTTGATGTGAAGTGCTTTG 4104
At2g03570 Y48F ATGAGAGAGAGCAAAAATCAAATCAATC Y48R CGCCTCCTCAGTAACTTCCAGC 267
At5g20430 Y51F ATGAAGGCTGGAAGATATGAGTATCG Y51R TGGTTTCAAAATAGTTTCCACGAGC 366
At5g20440 Y52F ATGGGTGGTTTTTTGGTTTTAAACAG Y52R TTGGTCCAACATAGTTTCCACTAGACT 651
At5g45550 Y53F ATGAGTCTCTTTGGGTTAGGAAGAAATC Y53R ATAAGGTGAAATGATAGATTCTATGAGCTCT 645
At1g69220 Y17F ATGGATCATAACTCGCCGAAATC Y17R GAGGCGGAGAATAGTGCGAAG 2508
At4g32830 Y31F ATGGCGATCCCTACGGAGAC Y31R TAACTCTGTAGATTCCAGAAGGATCAGC 882
At2g25880 Y32F ATGTTGTATCAGGCGGCTTCAG Y32R TTCCTCTGTAAAGGCCTGATGGG 846
At2g45490 Y33F ATGAGTAAGAAATCGACAGAATCTGACG Y33R TAATATCAATTGAGGCACACACACC 864
At5g41790 Y8F ATGAATAGTGCAGAGGAAGAGAACAAATC Y8R AGTTCGCTGCGACACTCTTCTTTG 3915
At1g73910 Y12F ATGTACGGTGGAGACGAAGTGTC Y12R GCATCGATAAAAGGTAGAGAGAAACC 435
At2g31300 Y19F ATGGCAGTGGTGGATGTTCATC Y19R AAACTGATTGCCAAGTTCTTGTTGC 1134
At2g30910 Y20F idem as Y19F Y20R AAACTGATTGCCAAGTTCTTGTTCC 1134
At2g19760 Y29F ATGTCTTGGCAATCATACGTCG Y29R GAGTTCAGACTCGATAAGGTAATCCC 393
At4g29350 Y30F ATGTCGTGGCAATCATACGTCG Y30R GAGACCAGACTCGATAAGGTAATCGC 393
At4g29340 Y23F ATGTCGTGGCAAACTTACGTTG Y23R GAGACCCTGTTCGAGGAGGTAATC 402
At2g19770 Y24F ATGTCGTGGCAAGCATACGTTG Y24R AAGACCCTGTTCGATCAAGTAATCAC 402
At4g26700 Y13F ATGTCTGGGTACGTGGGTGTTG Y13R TAAGTCCTTTTTCTTCCACACGAATG 2061
At5g48460 Y14F ATGTCAGGATTCGTCGGGATT Y14R TTCTATAGAGGAATCTGACGTAGAATCATC 1962
At2g04750 Y15F ATGTCTAGTTACGTTGGTGTTCTTGTCTC Y15R AACATCTGAAGATCCATCGTCTGTC 1956
At5g35700 Y16F idem as Y15F Y16R TGCATCTTTATTATTCTCACCATCAAC 2061
At1g70140 Y36F ATGGCTGCCATGTTTAATCATCC Y36R CATATCAGAATCCGACCCACCAG 2280
At2g43800 Y37F ATGACTACAATACCCTTCTGCTTCCTC Y37R GAACGAGCCATCGTCGTCG 2682
At1g24150 Y39F ATGCAGCCATGGCCACC Y39R CATATCGGAATCTGATCCACCC 2160
At5g54650 Y40F ATGGTTGGAATGATTCGAGGAGG Y40R GTCTGAATCTGAACTAGACTGATCCACG 2700
At5g67470 Y41F ATGAAAGCTCTTCAATCCAGATTCTTC Y41R CGTAGAAGAGTTGCTGCTGTGTTC 2697
At1g59910 Y42F ATGTCGTTTCTTTTCCGTAAGAACG Y42R AGATTCTGAGTCTGAGTCACTTGAACTG 2787
At3g25500 Y43F ATGCTCTTCTTCTTATTCTTCTTCTACTTACTC Y43R AGAAACTAATGAGATTGAGTTATGTTCGTC 3153
At5g67270 Y9F ATGGCTACGAACATTGGGATGATG Y9R TGCAGGTCAAGAGAGGAGATGAACC 987
At5g62500 Y10F ATGGCGACGAACATTGGGATG Y10R TAGTTTGGGTCTCTGCAGCAGC 879
At3g47690 Y11F ATGGCGACGAACATCGGAATG Y11R TGGCTTGAGTCTTTTCTTCTTCCAG 465
At5g04990 Y21F ATGTCGGCATCAACGGTGTC Y21R TTCACTTTCAGGTGAAGAGTCCTG 1413
At3g10730 Y22F ATGTCGGCGTCAACGGTG Y22R AGCATGAGCAACAGAGACTGAGTCT 1365
At4g34490 Y34F ATGGAAGAGGATTTGATTAAGCGC Y34R TTAGGCACCTGAATGCGAGAC 1431
At3g10220 Y44F ATGGCAACTTCGCGTTTACAG Y44R TATTTCATCTTCCTCGAAAGGGTC 720
Plant genes
At5g55230 A1F ATGGCAGTTACAGATACTGAAAGTCCT A1R TTGGTGAAGCTGGAACTTGATGAT 1761
At4g26760 A2F ATGGCAGTGACAGAAGCAGAAA A2R TACCATAGCTTGCACAAACAAAGTATG 1419
At2g01910 A3F ATGCTGGAAATTGGAAGTCCCA A3R TGCCTTGGAGAGGCGAGTCTG 1824
At5g51600 A4F ATGGCAAGTGTTCAAAAAGATCCG A4R TAACCAAACGACATTCAGACTGTAGC 2121
At1g27920 A5F ATGGGTTCGCTTCAGACACCG A5R TTGCCTCACTCTTTCTGCTTTCTC 1776
At2g38720 A6F ATGTCTCCGTCTTCAACCACTACTTG A6R TATCGTCTTTCTGAAGAGCAACATAGTT 1761
At3g60840 A7F ATGGGAGAGACTGAGGATGAAAAAG A7R TGCAAAAACCGGCCCTAACC 1944
At5g62250 A8F ATGTCCAAATCTCAAATCGAATCAAC A8R TGCCATGGCGTGATAGAGGAGT 1647
At1g14690 A9F ATGGCAAAATTTGTTGGTTTGG A9R TGAAATCACTGATGTCGGTATCTTCG 2121
At2g35630 A10F ATGTCGACGGAGGATGAGAAGTT A10R CATATGTTCCAGTGATCCACCTTTG 6063
At3g19960 A12F ATGTCTCAGAAGGTTACTCCATTCATG A12R ATACCTGGTGCTATTTCTCCTTCCC 3498
At4g27370 A14F ATGATGAAAAGTTCAGTGAAGGAAATTT A14R ATAACTTTTCTTGCACCACCAATTCC 3378
At1g08730 A15F ATGGTACGTGAATGTTTTACTTTCCTTAAC A15R CTCAGAGACTGGCAATAAGAAGCTAAATC 4716
At3g58160 A18F ATGGCTGAAAACATAATGGTGGATTC A18R AAAGTAATCTTCGAAGCCCCCTTC 3726
At3g51300 A19F ATGAGCGCTTCGAGGTTCG A19R TAGAATGGAGCATGCCTTCTGC 591
At1g20090 A20F ATGGCGTCAAGGTTTATAAAGTGTG A20R CAAGAACGCGCAACGGTTC 585
At2g17800 A21F ATGAGCGCTTCGAGGTTCATAAAG A21R CAAAATGGAGCAGGCTTTTTGTG 591
At1g75840 A22F ATGAGTGCTTCGAGGTTTATAAAGTGTG A22R CAAGAACACGCAGCGGTTCT 588
At4g35950 A23F ATGAGCGCATCAAGGTTCATAAAG A23R CAAGATGGAGCAGGCCTTTTG 591
At4g35020 A24F ATGAGTGCTTCAAGGTTTATCAAGTGT A24R GAGTATAGAACAACCTTTCTGAGATTTTCTC 594
At5g22330 A25F ATGGAGAAAGTAAAGATTGAAGAAATTCAG A25R TGAGATGTATTTTTCTTGTTGCTCATGC 1374
At4g03100 A26F ATGACTAAAGGCGGAGGTTGC A26R GTCGCTAGTGCTTGAGCTCAAAG 1272
At3g11490 A27F ATGGCTAAAGTTCTAAAATCGAGCC A27R TCGCCAAGCTTCGAATAGCTTC 1305
At1g08340 A28F ATGGGCCACATCCTTATTTGG A28R CGGGTTCGAGCTGCAAATG 1212
At3g55000 A29F ATGGACGATTATACAAGAGAGATGATGG A29R ATCTTTCCCTTCTTCTTCTTCACTTG 1011
At5g18580 A30F ATGTATAGCGGATCTAGCGATGGTG A30R CTGAGACTCTTCCTCAGGTGGTTC 1392
At2g42500 A31F ATGGGCGCGAATTCTATTCC A31R CAGGAAATAGTCTGGAGTCCTTCG 939
At3g58500 A32F ATGGGCGCGAATTCGCTT A32R GGAAATAGTTTGGGCAAAGACCTG 897
At1g69960 A33F ATGCCGCCGGCGACC A33R CAAAAAATAATCTGGAGTCTTGCGAG 921
At1g10430 TOB1F ATGCCGTCGAACGGAGATCT TOB1R CAAAAAATAATCAGGGGTCTTCCG 918
At3g05330 A34F ATGGTTGCAAGAACCCCACAG A34R AATCGCTTGGCAGCTACAAGC 1764
At3g61650 G-TUB1F ATGCCTCGGGAGATAATTACG G-TUB1R AGAACGCCAACTTCGGATCAAC 1425
At5g05620 G-TUB2F ATGCCGAGGGAGATAATTACG G-TUB2R AGAACGCTAACTTAGGATCCGC 1425
TAG genes
At2g27340 T1F ATGGTTGTTGTTTTTCTATCTCTGATTG T1R AGTGTATTCACATATGTATAGCTTGAAAACA 555
At3g61130 T2F ATGGCGCTAAAGCGAGGG T2R TTCATGAAGGTTGCAACGACG 2019
At4g39860 T3F ATGGAGAGAAACACGCCGG T3R GACCAATGAAATGCTGCTTTCAC 897
At2g44150 T4F ATGCCAGCCAGCAAAAAGATTC T4R TTTTGGTTTCTCAACTTCAAAGCA 1032
At5g44670 T5F ATGGCTAAAGAGAGAGACCAAAACAC T5R TTGTCTCGTCCTAAGCAAGCG 1557
At4g04200 T6F ATGGAAGAGAAGAAGACAGAATCCG T6R TCTCTTCTTCTTTGGTTCTCCTGC 375
At1g17030 T7F ATGAGAAACGATAACTTAAGGGTGGC T7R AATATCGGCATTTGGTATAACAATGTC 1365
At3g56490 T8F ATGGCTTCCGAGAAAGAAGCTG T8R ACCAGGAGGCCAGTTCATTTG 387
At5g11860 T9F ATGGAAGATGATGACTCTCCTTCTTC T9R CCTTTCGAAAGGATCTCCCG 915
At3g02420 T10F ATGGCGGAAGGCGGC T10R CCTGAACCACCATCTCTGAACG 1044
At4g24610 T11F ATGATCCCCAACGGAGAATTG T11R GTAGTAGAACGATTTGGCTTTATTATCTGC 3435
At5g47970 T13F ATGACAGTTTCAGAAGCTTATAGTCCAC T13R TTCCTCATCAAGAATCACAGGTCTC 1161
At1g35350 T14F ATGAAGTTTGGAAAAGAGTATGTTGCT T14R ACTATCTCGATCTTCCTCTTCATCG 2241
At4g09730 T15F ATGGTAGGAGCTTCAAGAACAATCC T15R TGAGATGTATTTTTCTTGTTGCTCATG 1488
At2g41830 T16F ATGAGCGCAGTCTCGGGTG T16R ACAGCCAGCAGCTTTAAGGAAG 2883
At4g28230 T17F ATGACATCAATTGAGGCAACAGAAAC T17R TTCTTCAAACTTCAGAAGCTGGC 1206
At3g61130 T18F ATGGCGCTAAAGCGAGGG T18R TTCATGAAGGTTGCAACGACAC 2019
At3g52180 T19F ATGAATTGTCTTCAGAATCTTCCCAG T19R AACTTCTGCCTCAGAACAAGTCTCC 1041
At3g06670 T20F ATGGAAGGGTTTCTTTTCTTCCA T20R GGATCCATTTACAGCCATTTCAG 2589
At1g17210 T21F ATGAAGGAAGAGGATGTGAGCTCG T21R CTTTTTCCCATGGCTGCTGA 2874
At3g01780 T22F ATGGACATTCTTTTTGCTCAGATCC T22R GTTAACTTTGGTATATTTTCTATCTTTGCAC 3576
At1g17690 T23F ATGACTTTGGGTTTTAATCTGCTCG T23R ACAGAAAGCAAACATGTTCTTCTCAG 2445
At1g18800 T24F ATGGTGACAGACAAGAGCAAGAAG T24R TTCCTCACCAACTTCGTCTTCTTC 765
At1g74560 T25F ATGGTCGCGGACAAGAGCA T25R TTCCTCACCATCTTCCTCCTCTT 768