Lorenzo et al., 2023 describes a gene discovery pipeline, denominated BREEDIT, which combines multiplex gene editing of large sets of genes with crossing schemes to improve complex traits such as yield and drought tolerance. Phenotyping the edited populations and multiplex amplicon sequencing of all target genes allowed for the identification of putative gene combinations that govern the traits of interest. The paper provides proof of concept data in maize but a similar approach can be used for many other crops.
