Chromatin Complex Isolation

Chromatin Complex Isolation iconAnalysis of sequence-specific protein-DNA interactions is critical for understanding how chromatin is organized and regulates genetic and epigenetic gene expression. We are developing an in situ method to detect protein-DNA interactions for a genomic region of interest under near-to-physiolocical conditions.

Many methods are available to identify genes targeted by a certain DNA-binding protein, including chromatin immunoprecipitation. From a gene-centered point-of-view however, in which the aim is to identify proteins binding to a certain genomic region of interest, mostly artificial approaches have been used so far, including yeast one-hybrid and electrophoretic mobility shift assay (EMSA). Experimental conditions in these artificial approaches are far from physiological, causing false negative results. Therefore, we are developing an in situ method to detect protein-DNA interactions for a genomic region of interest under near-to-physiolocical conditions.