Endocytosis and Brassinosteroid Signaling

endocytosis2Growing evidence suggests that plants have adapted endocytosis for signal transduction and use mechanisms similar to those of animals to regulate receptor internalization, because for example endocytic trafficking plays a role in the regulation of brassinosteroid signaling.
Despite progress in the description of some endocytic routes of plant plasma membrane proteins, we are far from a complete understanding of the endocytic trafficking of receptor complexes and to which extent their signaling activity requires and is modulated by these routes. The project aims at filling this gap by gaining basic knowledge of the relationship between the endocytic transport and signaling activity of brassinosteroid hormones. In addition, the discovery of novel plant endocytic and signaling scaffolds would give us an evolutionary insight into why certain gene families expanded in plants, while others range from being absent to having been maintained at an ancestral minimum.

Selected publications:

  • Di Rubbo S, Irani NG, Kim SY, Xu ZY, Gadeyne A, Dejonghe W, Vanhoutte I, Persiau G, Eeckhout D, Simon S, Song K, Kleine-Vehn J, Friml J, De Jaeger G, Van Damme D, Hwang I, Russinova E. (2013). The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid insensitive1 in Arabidopsis. Plant Cell. 25: 2986-2997.
  • Irani NG, Di Rubbo S, Mylle E, Van den Begin J, Schneider-Pizo? J, Hniliková J, Šíša M, Buyst D, Vilarrasa-Blasi J, Szatmári AM, Van Damme D, Mishev K, Codreanu MC, Kohout L, Strnad M, Caño-Delgado AI, Friml J, Madder A, Russinova E. (2012). Fluorescent castasterone reveals BRI1 signaling from the plasma membrane. Nat Chem Biol. 8: 583-589.
  • Di Rubbo S, Irani NG, Russinova E. (2011). PP2A phosphatases: the "on-off" regulatory switches of brassinosteroid signaling. Sci Signal. 4: pe25.
  • Irani NG, Russinova E. (2009) Receptor endocytosis and signaling in plants. Curr Opin Plant Biol. 12: 653-659.