Microscopy Infrastructure

Overview of all microscopes in the department

Confocal FV1000 Olympus (FV10 ASW)

This inverted confocal microscope has 405nm/458nm/488nm/559nm/633nm laser excitation lines. The system has two adjustable spectral emission window detectors and one filter based detector. The Fluoview software has a very user friendly interface for imaging multi area time lapses with individual image settings for each selected point. Available lenses include 10x, 20x dry, 40x oil and 60x water corrected.

This system is equipped with a time domain FLIM module (Picoquant) for ‘Fluorescence Lifetime Imaging Microscopy’. This module consists of a pulsed diode 440 nm laser, a time-correlated single-photon counting unit and a single-photon avalanche diode detector (SPAD) with a time resolution of 10 ps. The detector has three possible emission filter setups – a 480/40nm band pass for CFP emission, a 460LP and a 520/35-25 nm band pass filter for GFP. The Symphotime software controls the FLIM module and is synchronized with the confocal microscope.

 

Confocal TCS SP2 Leica

This upright confocal microscope is equipped with 405nm/458nm/488nm/559nm/633nm laser excitation lines. There is an adjustable spectral emission window on three detectors. Available lenses include 10x dry, 20x and 63x water corrected.

 

Confocal LSM 710 Zeiss

This inverted confocal microscope has 405nm/458nm/488nm/559nm/633nm laser excitation lines. There is an adjustable spectral emission window on two detectors. An extra slit in front of the UV emission can be inserted in the microscope allowing fast photoconversion of photoswitchable fluorophores on a small spot or a line. Available lenses include 10x, 20x dry, 40x and 63x water corrected.

 

Confocal LSM 5 Exciter Zeiss

This upright confocal microscope has 405nm/458nm/488nm/561nm/633nm laser excitation lines. Water dipping lenses allowing visualization without coverslips are available (20x, 40x and 63x). The microscope can be tilted horizontally to allow live visualization of specimens, such as actively growing Arabidopsis roots, within the gravity vector. Available lenses include 10x, 20x dry, 25x, 40x and 63x water corrected.

 

Confocal LSM510 Zeiss (Pascal)

This inverted confocal microscope has 458nm/488nm/543nm/633nm laser excitation lines. There are two filter based detectors and a DIC detector. Available lenses include 10x, 20x dry and 63x water corrected.

 

Spinning disc Ultraview Perkin Elmer

This inverted Nikon Ti microscope-based system has 405nm/440nm/488nm/515nm/561nm/640nm diode laser excitation lines controlled by the Volocity software. Two Hamamatsu 512x512 EMCCD cameras and a 509 and 580 dichroic kit allow simultaneous imaging of GFP/RFP or CFP/YFP. The microscope is equipped with a Perfect Focus System (PFSIII), reducing Z-drift, deal for long time imaging. Available lenses include 10x, 20x dry, 60x water and 100x oil corrected.

 

When speed is of superior demand rather than resolution, this system is the most convenient choice. Ratiometric imaging (Ca2+ sensors, phosphorylation sensors), 4D stitching and photokinesis FRAP on fly are examples of the possibilities that this system offers.

 

Epifluorescence microscope Axiovert 135M Zeiss

This is an inverted microscope with automated stage and shutters controlled by the Axiovision software, ideal for generating multi area time lapses and high content screening purposes. Filter sets are available for DAPI/CFP/GFP/YFP/RFP/GFP-RFP/CFP-YFP. Several objective lenses, up to 40x dry, are available. Available lenses include 10x, 20x dry, 40x dry and 40x oil corrected.

 

Epifluorescence microscope Axioimager M1 Zeiss

This is an upright microscope with manual stage controlled by the Axiovision software and Zen blue. Filter sets are available for DAPI/GFP/RFP/CFP-YFP/CFP-YFP-FRET. Several objective lenses up to 40x dry are available and also water dipping lenses (20x/40x/63x) can fitted onto this microscope for screening purposes. Available lenses include 10x, 20x dry, 40x dry and 40x oil corrected.

 

Epifluorescence microscope Axioskop Zeiss

This is an upright microscope with manual stage. Filter sets are available for DAPI/CFP/GFP/YFP/RFP/GFP-RFP/CFP-YFP. Available lenses include 10x, 20x dry and 40x oil corrected.


 

Macroscope MVX10 Olympus

This microscope has been tilted horizontally to allow low magnification visualization of fluorescent markers during gravistimulation experiments. It has automated shutters, although it is still equipped with a manual stage. This system allows to follow root growth during several (up to 40) hours, allowing to correlate fluorescent marker expression with root development and growth. Filter sets are available for GFP, YFP and RFP. Available lenses include 1x and 2x.

 

Stereomicroscopes MZFLIII (Leica GFP1) and MZ16F (Leica GFP2) Leica

Filter sets are available for GFP and RFP and also a very narrow band filter for GFP (only for the Leica GFP2). These microscopes mainly serve screening purposes.


 

Olympus ScanR

This inverted microscope is a high content screening platform, equipped with an automated stage, automated multi-channel imaging and autofocus. The software allows automated image analysis. Ideal for high throughput 96-well plate imaging.

 


TM-1000 Hitachi

This table-top SEM machine is user friendly and allows detailed observation of samples which are resistant against low vacuum conditions (e.g. pollen grains or trichomes). It is also widely used to scan nail-polish replica samples of dental-resin impressions of leave tissue to follow leaf growth over time (several days with one impression of a growing leaf each day).