- Through the VIB Genetic Service Facility: integrated high throughput robotic genetic screening platform, built around a Laboratory Information Management System (LIMS) and comprising:
- two 96-capillary sequencers (ABI3730XL, Applied Biosystems) for sequencing and microsatellite genotyping
- Roche FLX Genome Sequencer
- a MassARRAY Nanodispenser and Compact Analyzer (Sequenom) and a PSQTM HS96A System (Biotage) for SNP genotyping
- Biomek FX and NX robots for pre- and post-processing of templates
- Tools to conduct large-scale functional analyses of plant genes (see also the section gene function discovery)
- Design and production of spotted arrays for genome and transcriptome analysis
- Microarray analysis (Agilent, Affymetrix, Tiling; see also the Microarray Facility)
- Linkage mapping
- QTL analyses
- eQTL mapping
- Association mapping
- Allele-specific gene expression analysis
- Expression analysis using qRT-PCR
- Semi-automated RNA in situ localization in whole mount or tissue sections
- Identification of mutant genes by map-based cloning
- Chromatin Immunoprecipitation analysis
- DNA ploidy analysis
- Chemical genetics and the Compound Screening Facility
|