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"A researcher’s field guide to CRISPR in plants"

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Tuesday 24 January 2017, 14:00 - 15:30

 
ABSTRACT

It has been suggested that in the not-too-distant future, targeted genome editing with CRISPR will become as commonplace as performing a PCR. The simple design criteria and high efficacy in most biological systems has facilitated its adoption across a wide range of disciplines. However, some upfront thought is necessary to make the downstream workload as pain-free as possible. The Plant Genome Editing group at PSB is developing a number of cloning and analysis protocols that will facilitate genome editing experiments in plant systems. This seminar will cover gRNA design, cloning, and efficient methods to analyze gene editing outcomes. I will present the Golden Gateway® cloning system we are using to clone a collection of CRISPR vectors, how you might use the system for your own experiments, and the protocols and resources that are available. A comparison of mutant detection techniques will also be presented, with particular emphasis on TIDE, T7E1, and high-throughput amplicon sequencing. I will also discuss some of the alternative CRISPR technologies and look to the future of gene editing in plants.

Location Jozef Schell Seminar Room
Contact Prof Tom Jacobs
VIB, Dept. of Plant Systems Biology
Ghent University
Ghent
BELGIUM