Jacobs Thomas

Jacobs Thomas - Group leader
Joined the group in 2016

Thomas obtained his PhD from the University of Georgia with Wayne Parrott, developing several molecular tools for soybean biotechnology. In 2014 he moved to the lab of Greg Martin at the Boyce Thompson Institute for his postdoc. There he started developing CRISPR screens in plants. In 2016 he started the Plant Genome Editing group at the VIB-UGent Center for Plant Systems Biology. In 2022 he was appointed Associate Professor at the University of Gent. His group is focused on developing easy-to-use genome editing systems for all kinds of plant species, specifically focused on making large-scale gene knockouts and base editing.

39. Phosphoproteome analyses pinpoint the F‐box protein SLOW MOTION as a regulator of warm temperature‐mediated hypocotyl growth in Arabidopsis

Shanshuo Zhu, Lixia Pan, Lam Dai Vu, Xiangyu Xu, Beatriz Orosa‐Puente, Tingting Zhu, Pia Neyt, Brigitte van de Cotte, Thomas B. Jacobs, Joshua M. Gendron, Steven H. Spoel, Kris Gevaert, Ive De Smet

(2024) - New Phytologist

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38. Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts

Charlotte Cautereels, Jolien Smets, Jonas De Saeger, Lloyd Cool, Yanmei Zhu, Anna Zimmermann, Jan Steensels, Anton Gorkovskiy, Thomas B. Jacobs, Kevin J. Verstrepen

(2024) - Nature Communications

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37. Continual improvement of CRISPR‐induced multiplex mutagenesis in Arabidopsis

Ward Develtere, Ward Decaestecker, Debbie Rombaut, Chantal Anders, Elke Clicque, Marnik Vuylsteke, Thomas B. Jacobs

(2024) - The Plant Journal

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36. Identification and characterization of CYP71 subclade cytochrome P450 enzymes involved in the biosynthesis of bitterness compounds in Cichorium intybus

Charlotte De Bruyn, Tom Ruttink, Elia Lacchini, Stephane Rombauts, Annelies Haegeman, Ellen De Keyser, Christof Van Poucke, Sandrien Desmet, Thomas B. Jacobs, Tom Eeckhaut, Alain Goossens, Katrijn Van Laere

(2023) - Frontiers in Plant Science

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35. Ulva: An emerging green seaweed model for systems biology

Jonas Blomme, Thomas Wichard, Thomas B. Jacobs, Olivier De Clerck

(2023) - Journal of Phycology

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34. SMAP design: a multiplex PCR amplicon and gRNA design tool to screen for natural and CRISPR-induced genetic variation

Ward Develtere, Evelien Waegneer, Kevin Debray, Jonas De Saeger, Sabine Van Glabeke, Steven Maere, Tom Ruttink, Thomas B Jacobs

(2023) - Nucleic Acids Research

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33. Combining multiplex gene editing and doubled haploid technology in maize

Lennert Impens, Christian D. Lorenzo, Wout Vandeputte, Pieter Wytynck, Kevin Debray, Jari Haeghebaert, Denia Herwegh, Thomas B. Jacobs, Tom Ruttink, Hilde Nelissen, Dirk Inzé, Laurens Pauwels

(2023) - New Phytologist

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32. Continual improvement of multiplex mutagenesis in Arabidopsis

Ward Develtere, Ward Decaestecker, Debbie Rombaut, Chantal Anders, Elke Clicque, Marnik Vuylsteke, Thomas B. Jacobs

(2023) -

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31. BREEDIT: a multiplex genome editing strategy to improve complex quantitative traits in maize

Christian Damian Lorenzo, Kevin Debray, Denia Herwegh, Ward Develtere, lennert impens, Dries Schaumont, Wout Vandeputte, Stijn Aesaert, Griet Coussens, Yara De Boe, Kirin Demuynck, Tom Van Hautegem, Laurens Pauwels, Thomas B. Jacobs, Tom Ruttink, Hilde Nelissen, Dirk Inzé

(2023) - The Plant Cell

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30. Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform

Christophe Gaillochet, Alexandra Peña Fernández, Vera Goossens, Katelijn D’Halluin, Andrzej Drozdzecki, Myriam Shafie, Julie Van Duyse, Gert Van Isterdael, Camila Gonzalez, Mattias Vermeersch, Jonas De Saeger, Ward Develtere, Dominique Audenaert, David De Vleesschauwer, Frank Meulewaeter, Thomas B. Jacobs

(2023) - Genome Biology

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29. Mutually opposing activity of PIN7 splicing isoforms is required for auxin‐mediated tropic responses in Arabidopsis thaliana

Ivan Kashkan, Mónika Hrtyan, Katarzyna Retzer, Jana Humpolíčková, Aswathy Jayasree, Roberta Filepová, Zuzana Vondráková, Sibu Simon, Debbie Rombaut, Thomas B. Jacobs, Mikko J. Frilander, Jan Hejátko, Jiří Friml, Jan Petrášek, Kamil Růžička

(2022) - New Phytologist

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28. Conditional and tissue-specific approaches to dissect essential mechanisms in plant development

Marie Pfeiffer, Joanna Winkler, daniel van damme, Thomas B. Jacobs, Moritz K. Nowack

(2022) - Current Opinion in Plant Biology

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27. Simple and Efficient Modification of Golden Gate Design Standards and Parts Using Oligo Stitching

Jonas De Saeger, Mattias Vermeersch, Christophe Gaillochet, Thomas B. Jacobs

(2022) - ACS Synthetic Biology

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26. ROPGAP-dependent interaction between brassinosteroid and ROP2-GTPase signaling controls pavement cell shape in Arabidopsis

Cheng Zhang, Theresa Lauster, Wenxin Tang, Anaxi Houbaert, Shanshuo Zhu, Dominique Eeckhout, Ive De Smet, Geert De Jaeger, Thomas B. Jacobs, Tongda Xu, Sabine Müller, Eugenia Russinova

(2022) - Current Biology

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25. The heat is on: a simple method to increase genome editing efficiency in plants

Jonas Blomme, Ward Develtere, Ayse Köse, Júlia Arraiza Ribera, Christophe Brugmans, Jessica Jaraba-Wallace, Ward Decaestecker, Debbie Rombaut, Alexandra Baekelandt, Álvaro Daniel Fernández Fernández, Frank Van Breusegem, Dirk Inzé, Thomas Jacobs

(2022) - BMC Plant Biology

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24. Optimized Transformation and Gene Editing of the B104 Public Maize Inbred by Improved Tissue Culture and Use of Morphogenic Regulators

Stijn Aesaert, Lennert Impens, Griet Coussens, Els Van Lerberge, Rudy Vanderhaeghen, Laurence Desmet, Yasmine Vanhevel, Shari Bossuyt, Angeline Ndele Wambua, Mieke Van Lijsebettens, Dirk Inzé, Ellen De Keyser, Thomas B. Jacobs, Mansour Karimi, Laurens Pauwels

(2022) - Frontiers in Plant Science

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23. Mini-Review: Transgenerational CRISPR/Cas9 Gene Editing in Plants

Lennert Impens, Thomas B. Jacobs, Hilde Nelissen, Dirk Inzé, Laurens Pauwels

(2022) - Frontiers in Genome Editing

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22. A Simple and Low‐Tech Heat‐Shock Method to Increase Genome Editing Efficiency in Plants

Jonas Blomme, Júlia Arraiza Ribera, Ward Develtere, Thomas B. Jacobs

(2022) - Current Protocols

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21. Protoplast Preparation and Fluorescence-Activated Cell Sorting for the Evaluation of Targeted Mutagenesis in Plant Cells

Ward Decaestecker, Norbert Bollier, Rafael Andrade Buono, Moritz K. Nowack, Thomas B. Jacobs

(2022) - Methods in Molecular Biology

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20. Voices of biotech research

Nasim Annabi, Matthew Baker, Alistair Boettiger, Debojyoti Chakraborty, Yvonne Chen, Kizzmekia S. Corbett, Bruno Correia, James Dahlman, Tulio de Oliveira, Ali Ertuerk, Mehmet Fatih Yanik, Elizabeth Henaff, Meritxell Huch, Iliyan D. Iliev, Thomas Jacobs, Howard Junca, Albert Keung, Ilana Kolodkin-Gal, Smita Krishnaswamy, Madeline Lancaster, Evan Macosko, Mario Alberto Martínez-Núñez, Kyoko Miura, Jenny Molloy, Andrés Ochoa Cruz, Randall J. Platt, Avery D. Posey, Huilin Shao, Mijo Simunovic, Nikolai Slavov, Takanori Takebe, Luk H. Vandenberghe, Rajeev K. Varshney, Jianbin Wang

(2021) - Nature Biotechnology

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19. Efficient simultaneous mutagenesis of multiple genes in specific plant tissues by multiplex CRISPR

Norbert Bollier, Rafael Andrade Buono, Thomas B. Jacobs, Moritz K. Nowack

(2021) - Plant Biotechnology Journal

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18. A molecular toolkit for the green seaweed Ulva mutabilis

Jonas Blomme, Xiaojie Liu, Thomas B Jacobs, Olivier De Clerck

(2021) - Plant Physiology

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17. CRISPR screens in plants: approaches, guidelines, and future prospects

Christophe Gaillochet, Ward Develtere, Thomas B Jacobs

(2021) - The Plant Cell

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16. Efficient CRISPR-mediated base editing in Agrobacterium spp.

Savio Rodrigues, Mansour Karimi, lennert impens, Els Van Lerberge, Griet Coussens, Stijn Aesaert, Debbie Rombaut, Dominique Holtappels, Heba M. M. Ibrahim, Marc Van Montagu, Jeroen Wagemans, Thomas B. Jacobs, Barbara De Coninck, Laurens Pauwels

(2021) - Proceedings of the National Academy of Sciences

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15. GoldenGateway: A DNA Assembly Method for Plant Biotechnology

Mansour Karimi, Thomas B. Jacobs

(2021) - Trends in Plant Science

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14. Establishment of CRISPR/Cas9 Genome Editing in Witloof (Cichorium intybus var. foliosum)

Charlotte De Bruyn, Tom Ruttink, Tom Eeckhaut, Thomas Jacobs, Ellen De Keyser, Alain Goossens, Katrijn Van Laere

(2020) - Frontiers in Genome Editing

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13. CRISPR-Cas-Mediated Gene Knockout in Tomato

Gwen Swinnen, Thomas Jacobs, Laurens Pauwels, Alain Goossens

(2020) - Methods in Molecular Biology

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12. Genome Editing-Based Engineering of CESA3 Dual Cellulose-Inhibitor-Resistant Plants

Zhubing Hu, Teng Zhang, Debbie Rombaut, Ward Decaestecker, Aiming Xing, Sam D’Haeyer, Rene Höfer, Ilse Vercauteren, Mansour Karimi, Thomas Jacobs, Lieven De Veylder

(2019) - Plant Physiology

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11. CRISPR-TSKO: A Technique for Efficient Mutagenesis in Specific Cell Types, Tissues, or Organs in Arabidopsis

Ward Decaestecker, Rafael Andrade Buono, Marie L. Pfeiffer, Nick Vangheluwe, Joris Jourquin, Mansour Karimi, Gert Van Isterdael, Tom Beeckman, Moritz K. Nowack, Thomas B. Jacobs

(2019) - The Plant Cell

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10. DOF2.1 Controls Cytokinin-Dependent Vascular Cell Proliferation Downstream of TMO5/LHW

Wouter Smet, Iris Sevilem, Maria Angels de Luis Balaguer, Brecht Wybouw, Eliana Mor, Shunsuke Miyashima, Bernhard Blob, Pawel Roszak, Thomas B. Jacobs, Mark Boekschoten, Guido Hooiveld, Rosangela Sozzani, Ykä Helariutta, Bert De Rybel

(2019) - Current Biology

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9. POLAR-guided signalling complex assembly and localization drive asymmetric cell division

Anaxi Houbaert, Cheng Zhang, Manish Tiwari, Kun Wang, Alberto de Marcos Serrano, Daniel V. Savatin, Mounashree J. Urs, Miroslava K. Zhiponova, Gustavo E. Gudesblat, Isabelle Vanhoutte, Dominique Eeckhout, Sjef Boeren, Mansour Karimi, Camilla Betti, Thomas Jacobs, Carmen Fenoll, Montaña Mena, Sacco de Vries, Geert De Jaeger, Eugenia Russinova

(2018) - Nature

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8. Generation of a Collection of Mutant Tomato Lines Using Pooled CRISPR Libraries

Thomas B. Jacobs, Ning Zhang, Dhruv Patel, Gregory B. Martin

(2017) - Plant Physiology

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7. Simple gene silencing using the trans‐acting siRNA pathway

Thomas B. Jacobs, Noah J. Lawler, Peter R. LaFayette, Lila O. Vodkin, Wayne A. Parrott

(2016) - Plant Biotechnology Journal

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6. High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Thomas B. Jacobs, Gregory B. Martin

(2016) - Journal of Visualized Experiments

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5. A transgenic, visual screenable marker for soybean seeds

Lisa Kanizay, Thomas Jacobs, C. Nathan Hancock

(2016) - Transgenic Research

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4. Exploiting SNPs for biallelic CRISPR mutations in the outcrossing woody perennial Populus reveals 4‐coumarate:CoA ligase specificity and redundancy

Xiaohong Zhou, Thomas B. Jacobs, Liang‐Jiao Xue, Scott A. Harding, Chung‐Jui Tsai

(2015) - New Phytologist

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3. Compositions and methods of gene silencing in plants

Jacobs, Thomas B, Parrott, Wayne A, Vodkin, Lila O

(2015) -

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2. Targeted genome modifications in soybean with CRISPR/Cas9

Thomas B Jacobs, Peter R LaFayette, Robert J Schmitz, Wayne A Parrott

(2015) - BMC Biotechnology

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1. HtStuf: High‐Throughput Sequencing to Locate Unknown DNA Junction Fragments

Lisa B. Kanizay, Thomas B. Jacobs, Kevin Gillespie, Jade A. Newsome, Brittany N. Spaid, Wayne A. Parrott

(2015) - The Plant Genome

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