dissection of plant cytokinesis and phragmoplast structure: a survey of
Van Damme, François-Yves Bouget1, Kris Van Poucke, Dirk Inzé2, and
of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology,
Ghent University, Technologiepark 927, B-9052 Gent, Belgium
current address: Laboratoire Arago, UMR7628 CNRS, Université Pierre et Marie
Curie, BP 44, 66651 Banyuls sur Mer cedex, France
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identify molecular players implicated in cytokinesis and division plane
determination, the Arabidopsis thaliana genome was explored for potential
cytokinesis genes. More than 100 open reading frames were selected based on
similarity to yeast and animal cytokinesis genes, cytoskeleton and polarity
genes, and Nicotiana tabacum genes showing cell cycle controlled
expression. The subcellular localization of these proteins was determined by
means of GFP-tagging in tobacco Bright Yellow-2 (BY-2) cells and Arabidopsis
plants. Detailed confocal microscopy identified 15 proteins targeted to distinct
regions of the phragmoplast and the cell plate. EB1- and MAP65-like proteins
were associated with the plus-end, the minus-end, or along the entire length of
microtubules. The actin-binding protein myosin, the kinase Aurora, and a novel
cell cycle protein designated T22, accumulated preferentially at the midline.
EB1 and Aurora, in addition to other regulatory proteins (homologs of Mob1,
Sid1, and Sid2), were targeted to the nucleus, suggesting that this organelle
operates as a coordinating hub for cytokinesis.
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expressed under the 35S promotor unless otherwise listed.
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