ivsme

Ive De Smet leads the Functional Phosphoproteomics Group. To fully understand plant growth and development, we need to identify novel components and require insight in the underlying network. In the past, Arabidopsis research has mainly focused on transcriptional changes (Long et al. 2008 Annu Rev Cell Dev Biol 24:81), but there is an urgent need to gain insight in protein changes on different levels, including protein-protein interactions and post-translational protein modifications. With respect to the latter, temporary and reversible phosphorylation of proteins is essential in regulating intracellular biological processes. Phosphorylation affects protein folding (conformation), protein function and the regulation of enzymatic activities, defines substrate specificity, and influences protein localization, complex formation and degradation. The mechanistic importance of phosphorylation is obvious from its major influence on various cell functions, such as signal transduction, cell division, cell differentiation, and metabolic maintenance. During the last ten years, Arabidopsis thaliana has been proven to be an efficient model plant to study plant growth and development, but time has come to investigate signaling cascades in crop species such as maize, wheat and rice, species that are currently also under investigation.

Icon PhosphatasesKinasesWhile the knowledge on post-translational regulation through transient phosphorylation in plants is growing because of its crucial importance in plant molecular networks, it remains an underexplored and challenging area.

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Icon PhosphoproteomicsWe apply a gel-free phosphoproteomics pipeline to different biological systems: Arabidopsis cell suspension cultures, liquid Arabidopsis seedling cultures and the monocot leaves.

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