gejaeGeert De Jaeger leads the Functional Interactomics Group. Biological structures and functions are generally accomplished by networks of short- and long-term molecular interactions. As a discipline, functional interactomics aims to map system-wide molecular interactions and analyze their function. Initially focused on protein-protein interactions, the field is rapidly expanding towards other interaction types, such as those between proteins and nucleic acids, or between proteins and metabolites.
A major part of our research is to develop and fine tune interactomics tools for plant research, and more specifically for the discovery of protein-protein interactions and interactions between proteins and nucleic acids. At the core of our technology lies affinity purification of macromolecular complexes. The advantage of this approach is that interactions are discovered in situ.
These tools are applied to gather knowledge on cell growth and proliferation control in plants, and as such discover new approaches for the engineering of plant growth. Cell growth and proliferation play a key role in plant growth and development. We want to map protein complexes involved in the regulation of cell growth and proliferation and analyse their function. The tools to accomplish this are described in more detail below.

protein_isolationProtein-protein interactions as they appear in functional protein complexes have been studied in high throughput fashion in yeast, through yeast 2-hybrid on the one hand and affinity purification followed by mass spectrometry (AP-MS) on the other hand. We have developed a unique AP-MS platform for protein complex isolation from plant cells.

Read more ...

TAP iconWe offer the plant research community and companies access to our protein complex purification platform. Purifications under research service agreement are performed based on well-etablished protocols for complex purification from either the Arabidopsis cell suspension cultures PSB-D/L or Col-0 seedlings. We have recently been able to show that our complex purification technology performs equally well in crop species such as corn or rice.

Read more ...

Chromatin Complex Isolation iconAnalysis of sequence-specific protein-DNA interactions is critical for understanding how chromatin is organized and regulates genetic and epigenetic gene expression. We are developing an in situ method to detect protein-DNA interactions for a genomic region of interest under near-to-physiolocical conditions.

Read more ...